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Title 

Production of transgenic birdsfoot trefoil(Lotus corniculatus L.) plants by introduction of E35S promoter + AtNDPK2 gene

 

E35S 프로모터 + AtNDPK2 유전자 도입에 의한 버즈풋 트레포일 형질전환체 생산

Authors 

K Y KimY S JangG J ChoiB R SungW H KimS SeoB H LeeSang Soo Kwak

Issue Date 

2006

Citation 

Journal of Korean Society of Grassland Science, vol. 26, no. 2, pp. 83-90

Keywords 

birdsfoot trefoiltransgenic plantAtNDPK geneenvironmental stress

Abstract 

To develop transgenic birdsfoot trefoil (Lotus corniculatus L.) plants tolerant to environmental stress, Arabidopsis NDPK gene (AtNDPK) was introduced into birdsfoot trefoil plants using Agrobacteriummediated transformation and expressed powerfully under the control of the E35S promoter. The expression vector, pEN-K was used for introduction of AtNDPK gene into birdsfoot trefoil plants. The transformed calli were selected on kanamycin containing medium and then regenerated. The transformed birdsfoot trefoil plants were cultivated for 4 months on BOi2Y medium. Genomic DNA PCR and Southern blot analysis confirmed the incorporation of AtNDPK into the birdsfoot trefoil genome.

ISSN 

1013-9354

Link 

http://dx.doi.org/10.5333/KGFS.2006.26.2.083

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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