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Title 

Detection of surface asialoglycoprotein receptor expression in hepatic and extra-hepatic cells using a novel monoclonal antibody

Authors 

Jung-Hyun ParkK L KimEun Wie Cho

Publisher 

Springer Verlag (Germany)

Issue Date 

2006

Citation 

Biotechnology Letters, vol. 28, no. 14, pp. 1061-1069

Keywords 

asialoglycoprotein receptorflow cytometryhuman peripheral blood lymphocytemonoclonal antibodyT cellscellsRNAanimal

Abstract 

The asialoglycoprotein receptor (ASGPR) is a heterodimeric membrane protein which is involved in the internalization of desialylated glycoproteins and also in the binding and uptake of various pathogenic viruses. To facilitate the analysis of ASGPR expression, we generated a monoclonal antibody, termed ASSA-1, that is specific to the ASGPR H1 subunit based on ELISA and Western blots analysis. ASSA-1 also reacted to surface-displayed ASGPR in live cells thus enabling analysis of ASGPR expression by immunofluorescence flow cytometry, which we used to analyze established human liver cell lines previously confirmed to be positive for ASGPR mRNA expression. In agreement with previous reports, surface ASGPR was also detected in extra-hepatic cells and, surprisingly, even in human T cell lines, which was then further confirmed in activated, but not in resting, primary human peripheral blood lymphocytes. These observations suggest that ASGPR has a broad pattern of expression that even extends into cells from the immune system, which biological meanings still have to be analyzed. We expect that monoclonal antibody ASSA-1 will serve as a new powerful tool in analyzing the biological role of ASGPR in hepatic and extra-hepatic cells.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-006-9064-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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