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Title 

Identification of the down-regulated genes in a mat1-2-deleted strain of Gibberella zeae, using cDNA subtraction and microarray analysis

Authors 

S H LeeSanghyeob LeeD ChoiY W LeeS H Yun

Publisher 

Elsevier

Issue Date 

2006

Citation 

Fungal Genetics and Biology, vol. 43, no. 4, pp. 295-310

Keywords 

cDNA microarraygibberella zeaeMAT1-2sexual reproductionsuppressive subtractive hybridizationcomplementary DNAascomycetesdeletion mutantDNA microarraydown regulation

Abstract 

Gibberella zeae (anamorph: Fusarium graminearum), a self-fertile ascomycete, is an important pathogen of cereal crops. Here, we have focused on the genes specifically controlled by the mating type (MAT) locus, a master regulator of sexual developmental process in G. zeae. To identify these genes, we employed suppression subtractive hybridization between a G. zeae wild-type strain Z03643 and the isogenic self-sterile mat1-2 strain T43ΔM2-2. Both reverse Northern and cDNA microarray analyses using 291 subtractive unigenes confirmed that 58.8% (171 genes) were significantly down-regulated in T43ΔM2-2. Among these, 98 could be either manually or automatically annotated based on known functions of their possible homologs. Northern blot analysis revealed that all of the genes examined were differentially regulated by MAT1-2 during sexual development. This study is the first report on the set of genes that are transcriptionally altered by the deletion of MAT1-2 during sexual reproduction in G. zeae.

ISSN 

1087-1845

Link 

http://dx.doi.org/10.1016/j.fgb.2005.12.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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