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Title 

Focal adhesion and actin organization by a cross-talk of TM4SF5 with integrin α2 are regulated by serum treatment

Authors 

S Y LeeY T KimM S LeeY B KimE ChungSemi KimJ W Lee

Publisher 

Elsevier

Issue Date 

2006

Citation 

Experimental Cell Research, vol. 312, no. 16, pp. 2983-2999

Keywords 

actin organizationfocal adhesion kinasegrowth factorintegrinTM4SF5actinalpha2 integrinmembrane proteinpaxillinprotein antibody

Abstract 

The biological functions of transmembrane 4 L6 family member 5 (TM4SF5) homologues to a tumor-associated antigen L6 are unknown, although it is over-expressed in certain forms of cancer. In the present study, the ectopic expression of TM4SF5 in Cos7 cells reduced integrin signaling under serum-containing conditions, but increased integrin signaling upon serum-free replating on substrates. TM4SF5 regulated actin organization and focal contact dynamics via the serum treatment-dependent differential regulation of FAK Tyr925 and paxillin Tyr118 phosphorylations and their localizations on peripheral cell boundaries. Y925F FAK mutation abolished the TM4SF5 effects. TM4SF5 associated with integrin α2 subunit, and this association was abolished by serum treatment. Furthermore, functional blocking anti-integrin α2 antibody abolished TM4SF5-enhanced signaling activity and caused membrane blebbing with abnormal actin organization. TM4SF5 increased chemotactic but decreased haptotactic migration. Altogether, this study reveals the functions of TM4SF5 collaborative with integrin signaling to alter focal contact dynamics, actin reorganization, and migration. Furthermore, this study suggests a mechanism of cross-talk between TM4SF5 and integrin which is further regulated by growth factor signaling.

ISSN 

0014-4827

Link 

http://dx.doi.org/10.1016/j.yexcr.2006.06.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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