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Title 

Simple method for RNA preparation from cyanobacteria

Authors 

Byung-Hyuk KimHee-Mock OhYoung Ki LeeGang Guk ChoiChi-Yong AhnByung Dae YoonHee-Sik Kim

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

Journal of Phycology, vol. 42, no. 5, pp. 1137-1141

Keywords 

bead-phenol-chloroform methodcyanobacteriaRNA extractionxanthogenate-SDS-phenol methodRNA

Abstract 

A simple and rapid method is presented for the preparation of RNA from various cyanobacteria. Unlike other methods that require a lysis solution, lysozymes, or proteinase K, the proposed method, called the bead-phenol- chloroform (BPC) method, uses silica/zirconia beads, phenol, and chloroform to break the cells and extract RNA more efficiently. Experiments confirm that the BPC method can successfully isolate total RNA from various cyanobacterial strains without DNA contamination, and the extracted RNA samples have a relatively high purity, concentration, and yield. Furthermore, the BPC method is more rapid, simple, and economical when compared with previously reported methods.

ISSN 

0022-3646

Link 

http://dx.doi.org/10.1111/j.1529-8817.2006.00263.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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