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Title 

Gene disruption using In vivo and In vitro methylation in Streptomyces griseus

Authors 

J S MaengKyung Sook BaeJangryul Kwak

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2006

Citation 

Journal of Microbiology and Biotechnology, vol. 16, no. 9, pp. 1472-1476

Keywords 

gene disruptionhomologous recombinationmethylationstreptomyces griseustransformationDNA methylationgene transfergene vectorgenetic transformationin vitro study

Abstract 

Previous study demonstrated that the restriction barrier of Streptomyces griseus is almost completely bypassed by the Streptomyces-E. coli shuttle vectors passed through the E. coli GM161 strain and methylated with AluII and HpaII methyltransferases. The same DNA methylation of the genomic DNA fragments cloned the nonreplicative vectors generated integrative transformation and gene disruption of their chromosomal counterparts at high efficiencies in S. griseus. This result indicated that the efficiency of gene disruption depends on the efficient transfer of the incoming DNA into bacterial hosts.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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