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Title 

Investigation of the doughnut effect in Cy3-Labeled protein microarrays using scanning near-field optical microscope

Authors 

A GokarnaY H KimY H ChoM S LeeI C KangHyun Kyu ParkMin-Gon KimBong Hyun Chung

Publisher 

Springer Verlag (Germany)

Issue Date 

2006

Citation 

Optical Review, vol. 13, no. 4, pp. 288-291

Keywords 

confocal microscopyfluorescenceprotein chipprotein microarrayscanning near field optical microscopy

Abstract 

We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known "doughnut effect" observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image.

ISSN 

1340-6000

Link 

http://dx.doi.org/10.1007/s10043-006-0288-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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