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Title | Dykellic acid inhibits cell migration and tube formation by RhoA-GTP expression |
| 다이켈릭산이 RhoA-GTP 발현에 의해 세포이동과 tube 형성을 저해 |
Authors | J C Heo; J Y Park; S U Woo; J R Rho; Ho Jae Lee; Sung Uk Kim; Yung Hee Kho; S H Lee |
Publisher | Pharmaceutical Society of Japan |
Issue Date | 2006 |
Citation | Biological & Pharmaceutical Bulletin, vol. 29, no. 11, pp. 2256-2259 |
Keywords | angiogenesis; cell migration; dykellic acid; invasion; RhoA; RhoA-GTP; RhoA guanine nucleotide binding protein; animal cell; cell invasion |
Abstract | Dykellic acid, a novel factor initially identified from the culture broth of Westerdykella multispora F50733, has been shown to inhibit matrix metalloprotease 9 activity, caspase-3 activity, B cell proliferation and LPS-induced IgM production, suggesting that this factor may have anti-cancer effects. In an effort to further address the possible anti-tumoral effects of dykellic acid, we used wound healing, invasion and RhoA-GTP assays to examine the effects of dykellic acid on cell migration, invasion and angiogenesis. Our results revealed that dykellic acid dose-dependently inhibits B16 cell migration and motility, and inhibits HUVEC tube formation. Western blot analysis of the active form of RhoA (RhoA-GTP) showed that dykellic acid treatment decreased the levels of RhoA-GTP. These findings collectively suggest that dykellic acid may have both anti-metastatic and antiangiogenic acitivites, and provides the first evidence for the involvement of RhoA in dykellic acid-induced effects. |
ISSN | 0918-6158 |
Link | |
Appears in Collections | |
Registered Date |
2017-04-19 |
| Files in This Item: | Size | Format | |
|---|---|---|---|
| 6678.pdf | 764Kb | Adobe PDF | 
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