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Title 

Characterization and selection of lactic acid bacteria producing β-galactosidase

 

β-galactosidase 생산 유산균 선별 및 특성 조사

Authors 

Young Ki LeeS ChoiY I ParkChan Sun ParkByung Dae YoonY S HwangHee-Sik Kim

Publisher 

The Microbiological Society of Korea

Issue Date 

2006

Citation 

Korean Journal of Microbiology, vol. 42, no. 3, pp. 216-222

Keywords 

β-galactosidaselactobacillus내담즙성내산성유산균

Abstract 

This study was carried out to select the lactic acid bacteria producing β-galactosidase (lactase) and investigate the properties of the β-galactosidase. About 100 strains of lactic acid bacteria showing blue colony on the MRS agar medium containing X-gal were isolated from several kinds of Kimchi. Among them, 2 strains were selected as potential β-galactosidase producers. The selected strains, ET-1 and LA-12, were identified as Lactobacillus fermentum and L. acidophilus, respectively by the analysis of 16S rDNA sequences. They showed relatively high β-galactosidase activity and cellular viability. Their β-galactosidase showed the highest activity at 55oC. And the optimum pHs of the enzymes produced by ET-1 and LA-12 were pH 5.5 and pH 7.0, respectively. They were also highly resistant to artificial gastric juice and bile. Two selected strains showed little change of viable cell number for 3 hr incubation in artificial gastric juice, and maintained the viable cell number at 108 CFU/ml for 24 hr in 0.3% oxgall after incubation for 2 hours in artificial gastric juice. Based on these results, ET-1 and LA-12 are expected to be applied in dairy industry.

ISSN 

0440-2413

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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