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Title 

Gigantol isolated from the whole plants of Cymbidium goeringii inhibits the LPS-induced iNOS and COX-2 expression via NF-κB inactivation in RAW264.7 macrophages cells

Authors 

J H WonJ Y KimK J YunJ H LeeN I BackH G ChungS A ChungTae Sook JeongM S ChoiK T Lee

Publisher 

Georg Thieme Verlag

Issue Date 

2006

Citation 

Planta Medica, vol. 72, no. 13, pp. 1181-1187

Keywords 

cyclooxygenase-2cymbidium goeringiigigantolinducible nitric oxide synthaseNF-κBorhidaceaecymbidium goeringii extractimmunoglobulin enhancer binding proteininterleukin 1betaplant extract

Abstract 

During our efforts to find bioactive natural products with anti-inflammatory activity, we isolated gigantol from the whole plants of Cymbidium goeringii (Orchidaceae) by activity-guided Chromatographic fractionation. Gigantol was found to have potent inhibitory effects on LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 cells. Consistent with these findings, gigantol suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in RAW 264.7 cells in a concentration-dependent manner. Our data also indicate that gigantol is a potent inhibitor of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) release and influenced the mRNA expression levels of these cytokines in a dose-dependent manner. Furthermore, a reporter gene assay for nuclear factor kappa B (NF-κB) and an electromobility shift assay (EMSA) demonstrated that gigantol effectively inhibited the activation of NF-κB, which is necessary for the expression of iNOS, COX-2, TNF-α, IL-1β and IL-6. Thus, our studies suggest that gigantol inhibits LPS-induced iNOS and COX-2 expression by blocking NF-κB activation.

ISSN 

0032-0943

Link 

http://dx.doi.org/10.1055/s-2006-947201

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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