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Title 

Oral immunogenicity of potato-derived HBsAg middle protein in BALB/c mice

Authors 

Jung-Won YoumYoung Suk WonJae Heung JeonC J RyuYang Kyu ChoiHyoung-Chin KimB D KimHyouk JoungHyun Soon Kim

Publisher 

Elsevier

Issue Date 

2007

Citation 

Vaccine, vol. 253, no. 3, pp. 577-584

Keywords 

HBVoral immunizationplant-derived vaccinePreS2 + S regionshepatitis B surface antigenhepatitis B vaccineM proteinprotein antibodyrecombinant vaccinevirus protein

Abstract 

The antibodies to preS2 synthetic peptides have been probed to neutralize hepatitis B virus (HBV), and also the addition of preS2 sequence could enhance the antibody response compared with a conventional vaccine in the non- and low responders. Previously, we generated transgenic potatoes expressing middle protein, which contains additional 55 amino acid preS2 region at the N-terminus of the S protein, of HBV to determine the feasibility of developing a plant-delivered HBV vaccine. In this study, we monitored the immune response after induction of immunoglobulin by boosting and assessed the efficacy of the mucosal immune response with regard to generate IgA antibodies. The HBsAg middle protein expressed in our transgenic potatoes was well immunized at low antigenic quantities in mice and the induced anti-S or anti-preS2 antibodies were sustained for the whole period without decrease. Orally delivery of plant-derived HBsAg middle protein to mice resulted in fecal anti-S or anti-preS2 as well as serum IgG. In addition, we used antibodies induced from the immunized mice with the potato-derived rHBsAg in competition assay as competitors to confirm the binding ability of preS2 antibodies to surface antigen of hepatitis virus. Anti-preS2 antibodies induced from immunized mice with transgenic potatoes effectively competed with anti-preS2 murine antibody H8 as expected. From these results, the inclusion of preS2 antigen to HBV plant vaccine may provide additional protective immunity in the HBV prevention.

ISSN 

0264-410X

Link 

http://dx.doi.org/10.1016/j.vaccine.2006.05.131

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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