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Title 

High-yield production of functional human lactoferrin in transgenic cell cultures of Siberian ginseng(Acanthopanax senticosus)

Authors 

S H JoSuk Yoon KwonDoo Sang ParkKyoung Sil YangJ W KimK T LeeSang Soo KwakHaeng Soon Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2006

Citation 

Biotechnology and Bioprocess Engineering, vol. 11, no. 5, pp. 442-448

Keywords 

cell culturehigh expression promoterhuman lactoferrinmedicinal plantrecombinant proteinginseng extractlactoferrincell growthhumannonhuman

Abstract 

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hlf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhlf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/BF02932312

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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