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Title 

Monoclonal anti-thrombopoietin antibodies generated by genetic immunization

Authors 

N K LimJ H KimS Y KimH J KangK S KimS LeeHyo Jeong HongK S Inn

Publisher 

Mary Ann Liebert

Issue Date 

2006

Citation 

Hybridoma, vol. 25, no. 2, pp. 75-79

Keywords 

monoclonal antibodymonoclonal antibody 2B9A10monoclonal antibody 4C16B15thrombopoietinthrombopoietin antibodyantibody productionimmunizationantibodies, monoclonalantibody formation

Abstract 

Thrombopoietin (TPO) is a megakaryocyte growth and differentiation factor that is currently being investigated as a therapeutic for cancer patients undergoing myelosuppressive chemotherapy. We generated monoclonal antibodies (MAbs) specific for human thrombopoietin (hTPO) by genetic immunization using an hTPO expression plasmid and an adjuvant plasmid that encodes mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). All genetically immunized mice exhibited a high humoral immune response. Splenocytes from these mice were used to generate hybridomas. Two MAbs, designated 2B9A10 and 4C16B15 (of IgG1 and IgG3 isotypes, respectively), were subsequently selected and produced. They specifically recognized and precipitated recombinant hTPO produced by mammalian cells and were effective in sandwich enzyme-linked immunosorbent assays (ELISAs) for hTPO quantitation. Our results demonstrate that these MAbs should be useful for purification and quantitation of hTPO in clinical and laboratory settings.

ISSN 

0272-457X

Link 

http://dx.doi.org/10.1089/hyb.2006.25.75

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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