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Title 

Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells

Authors 

D H KwakKweon YuS M KimD H LeeJ U JungJ W SeoN KimS LeeK Y JungH K YouH A KimY K Choo

Publisher 

Korean Society of Medical Biochemistry

Issue Date 

2006

Citation 

Experimental and Molecular Medicine, vol. 38, no. 6, pp. 668-676

Keywords 

cell differentiationembryonic developmentfluorescent antibody techniquesgangliosidesmesenchymal stem cellsstem cellsanimal cellcell proliferationembryonic stem cell

Abstract 

Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjugated glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. However, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies. In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were expressed. Results from immunostaining were consistent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of gangliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.

ISSN 

1226-3613

Link 

http://dx.doi.org/10.1038/emm.2006.79

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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