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Title 

Prohibitin is involved in mitochondrial biogenesis in plants

Authors 

C S AhnJeong Hee LeeA R HwangW T KimH S Pai

Publisher 

Wiley-Blackwell

Issue Date 

2006

Citation 

Plant Journal, vol. 46, no. 4, pp. 658-667

Keywords 

mitochondrial morphologyreactive oxygen speciessenescencestress tolerancevirus-induced gene silencingplants (botany)prohibitinmitochondrial membraneplant proteinsplants

Abstract 

Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell-cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus-induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana, designated NbPHB1 and NbPHB2. NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10- to 20-fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress-inducing reagents, including H2O2, paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.

ISSN 

0960-7412

Link 

http://dx.doi.org/10.1111/j.1365-313X.2006.02726.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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