상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Oriented immobilization of antibodies with GST-fused multiple Fc-specific B-domains on a gold surface

Authors 

Tai-Hwan HaS O JungJeong Min LeeKun Yeong LeeY LeeJ S ParkBong Hyun Chung

Publisher 

American Chemical Society

Issue Date 

2007

Citation 

Analytical Chemistry, vol. 79, no. 2, pp. 546-556

Keywords 

antigenschemical modificationderivativesgoldsurface plasmon resonanceglutathioneimaging microarraysmaleimide groupantibodiesamine

Abstract 

We have constructed a novel platform for the oriented buildup of immunoglobulins on a gold surface for a surface plasmon resonance imaging microarray. To this end, genetically engineered glutathione S-transferase proteins bearing one, two, and three Fc-specific B-domains in protein G from Streptococci (GST-GB1, -GB2, and -GB3, respectively) were produced. In order to tether these GST-GBx proteins specifically, a novel glutathione-derivatized ligand (LA-GSH) was also synthesized from a biaminated tri(ethylene glycol) backbone. Each end of the backbone was further functionalized with a maleimide group for a glutathione modification and a lipoic acid for surface immobilization. The glutathione ligand demonstrated a negligible nonspecific protein adsorption toward other spectator proteins while showing a strong specific association toward GST-GBx proteins. This Fc-specific surface exhibited at least a 2-fold enhancement in the immunoglobulin density (from human and mouse) with its antigen capture capability totally conserved compared to a covalentiy tethered GBx proteins. A single antibody tethered on the GST-GB 3 is estimated to capture two antigens (enhanced green fluorescent protein), and this antigen capture ratio seems to be the most efficient value ever observed.

ISSN 

0003-2700

Link 

http://dx.doi.org/10.1021/ac061639+

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)