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Title 

Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae

Authors 

J Y ChoiJungoh AhnS I KimH J Shin

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2006

Citation 

Journal of Microbiology and Biotechnology, vol. 16, no. 12, pp. 2000-2003

Keywords 

α-Glucosidaseprotein expressionrecombinant enzymesaccharomyces cerevisiaethermus caldosphilus Gk24alpha glucosidasegene expressiongene expression regulationthermus caldophilus

Abstract 

A gene (GenBank AF096282) coding for a α-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/ mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/ mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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