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Title 

Novel candidate targets of Wnt/β-catenin signaling in hepatoma cells

Authors 

Heun-Sik LeeMee Hee ParkSuk-Jin YangKyung Chan ParkNam-Soon KimYong Sung KimD I KimHyang Sook YooE J ChoiYoung Il Yeom

Publisher 

Elsevier

Issue Date 

2007

Citation 

Life Sciences, vol. 80, no. 7, pp. 690-698

Keywords 

cDNA microarrayHCCtarget genesWnt/β-cateninbeta cateninWnt proteincell adhesioncell growthcell proliferationgene targeting

Abstract 

The activity of β-catenin/TCF, the key component of Wnt signaling pathway, is frequently deregulated in HCC, resulting in the activation of genes whose dysregulation has significant consequences on tumor development. Therefore, identifying the target genes of Wnt signaling is important for understanding β-catenin-mediated carcinogenesis. We analyzed the transcriptome profile of human hepatoma cell lines using cDNA microarrays representing 15,127 unique, liver-enriched gene loci to identify the target genes of β-catenin-mediated transcription (p < 0.005). This analysis yielded 130 potential Wnt-associated classifier genes, and we found 33 of them contain consensus TCF-binding sites in presumptive transcriptional regulatory sequences. These genes were, then, tested for their Wnt-dependence of expression in experimental models of Wnt activation. Genes such as RPL29, NEDD4L, FUT8, LYZ, STMN2, STARD7 and KIAA0998 were proven to be up-regulated upon Wnt/β-catenin activation. Gene ontology analysis of the 33 candidate genes indicated the presence of functional categories relevant to Wnt pathway such as cell growth, proliferation, adhesion and signal transduction. In conclusion, we identified a number of candidate Wnt/β-catenin target genes that can be useful for studying the role of altered Wnt signaling in liver cancer development, and showed that some of them might be direct targets of Wnt signaling in hepatoma cells.

ISSN 

0024-3205

Link 

http://dx.doi.org/10.1016/j.lfs.2006.10.024

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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