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Title 

Monitoring of microbial diversity and activity during bioremediation of crude oil-contaminated soil with different treatments

Authors 

Kyung-Hwa BaekByung Dae YoonByung-Hyuk KimDae-hyun ChoI S LeeHee-Mock OhHee-Sik Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2007

Citation 

Journal of Microbiology and Biotechnology, vol. 17, no. 1, pp. 67-73

Keywords 

alkB genebioaugmentationbiostimulationbiosurfactantdenaturing gradient gel electrophoresismicrobial communitybioremediationmicrobial attenuationmicrobial degradationmicrobial diversity

Abstract 

The present study compared the microbial diversity and activity during the application of various bioremediation processes to crude oil-contaminated soil. Five different treatments, including natural attenuation (NA), biostimulation (BS), biosurfactant addition (BE), bioaugmentation (BA), and a combined treatment (CT) of biostimulation, biosurfactant addition, and bioaugmentation, were used to analyze the degradation rate and microbial communities. After 120 days, the level of remaining hydrocarbons after all the treatments was similar, however, the highest rate (k) of total petroleum hydrocarbon (TPH) degradation was observed with the CT treatment (P<0.05). The total bacterial counts increased during the first 2 weeks with all the treatments, and then remained stable. The bacterial communities and alkane monooxygenase gene fragment, alkB, were compared by denaturing gradient gel electrophoresis (DGGE). The DGGE analyses of the BA and CT treatments, which included Nocardia sp. H17-1, revealed a simple dominant population structure, compared with the other treatments. The Shannon-Weaver diversity index (H′) and Simpson dominance index (D), calculated from the DGGE profileg using 16S rDNA, showed considerable qualitative differences in the community structure before and after the bioremediation treatrnent as well as between treatment conditions.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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