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Title | Analysis of recombinant protein expression using localized surface plasmon resonance (LSPR) |
Authors | Yong Beom Shin; Jeong-Min Lee; M R Park; Min-Gon Kim; Bong Hyun Chung; H B Pyo; S Maeng |
Publisher | Elsevier |
Issue Date | 2007 |
Citation | Biosensors & Bioelectronics, vol. 22, no. 9, pp. 2301-2307 |
Keywords | affinity-tagged protein; attenuated total reflection; gold nano-island; label-free assay; localized surface plasmon resonance (LSPR); well chip; proteins; surface plasmon resonance; affinity tagged protein; protein analysis |
Abstract | The localized surface plasmon resonance (LSPR)-based optical biosensor using nano-structures of noble metals has been considered as a useful tool for label-free detection of DNA hybridization and protein-protein interactions. We fabricated LSPR-based optical biosensors using gold nano-islands (nominal thickness; 75 ?) on glass substrates that were easily made using the conventional fabrication methods. The formation of gold nano-islands on glass substrates was realized by heat treatment of thin gold film deposited with a low deposition rate (∼0.05 ?/s). The morphologies of sensor surfaces composed of gold nano-islands were observed using an atomic force microscope (AFM) with a non-contact mode. To investigate the sensing capacity of the gold nano-island sensor for the binding of proteins by affinity interactions, the streptavidin and biotin interaction was used as a model system. In addition, detection of recombinant glutathione-S-transferase (GST)-tagged human interleukin-6 (hIL6) expressed in Escherichia coli was carried out by LSPR. It is expected that the LSPR sensors composed of gold nano-islands can be an alternative to traditional methods such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for fast analysis of protein expression. |
ISSN | 0956-5663 |
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Registered Date |
2017-04-19 |
