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Title 

Translation elongation factor 1-α gene from Pichia pastoris: Molecular cloning, sequence, and use of its promoter

Authors 

Jungoh AhnJiyeon HongHyeok Won LeeMyongsoo ParkEun Gyo LeeChun Sug KimEui Sung ChoiJoonki JungHong-Weon Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Applied Microbiology and Biotechnology, vol. 74, no. 3, pp. 601-608

Keywords 

constitutive promoterpichia pastoristranslation elongation factorgenesmolecular dynamicsgene encodingcloningtranslation elongation factor 1 alpha

Abstract 

The gene encoding translation elongation factor 1-α from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P TEF1 ) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P GAP ) by using a bacterial lipase gene as a reporter gene. P TEF1 demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P GAP . The sequence of the gene was deposited in GenBank under accession no. EF014948.

ISSN 

0175-7598

Link 

http://dx.doi.org/10.1007/s00253-006-0698-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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