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Title 

Transduced PEP-1-Grb7 fusion protein suppressed LPS-induced COX-2 expression

Authors 

J J AnS Y KimS H LeeD W KimH J RyuS I YeoS H JangH J KwonT Y KimSang Chul LeeHaryoung PooS W ChoK S LeeJ ParkW S EumS Y Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Journal of Biochemistry and Molecular Biology, vol. 40, no. 2, pp. 189-195

Keywords 

atopic dermatitis (AD)COX-2PEP-1-Grb7protein therapyprotein transductioncyclooxygenase 2growth factor receptor bound protein 7hybrid proteinPep 1 peptidepeptide

Abstract 

Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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