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Title 

Identification of Novel regulators of apoptosis using a high-throughput cell-based screen

Authors 

K M ParkE KangYeo-Jin JeonN KimNam-Soon KimHyang Sook YooYoung Il YeomSoo Jung Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Molecules and Cells, vol. 23, no. 2, pp. 170-174

Keywords 

apoptosisautomated fluorescence microscopehypothetical genessubcellular imagingcaspase 7complementary DNADNA fragmentnicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferaseautomation

Abstract 

High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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