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Title 

Yeast-based screening to identify modulators of G-protein signaling using uncontrolled cell division cycle by overexpression of Stm1

Authors 

Kyung Sook ChungMi Sun WonJung Joon LeeJiwon AhnKwang Lae HoeDong Uk KimK B SongHyang Sook Yoo

Publisher 

Elsevier

Issue Date 

2007

Citation 

Journal of Biotechnology, vol. 129, no. 3, pp. 547-554

Keywords 

GPCRsmitotic haploidizationschizosaccharomyces pombeStm1yeast-based screeningcell proliferationgene expressionyeastcell division cycledrug screening systems

Abstract 

Stm1, a G-protein coupled receptor, which senses nutritional state drives cells to stop the proliferative cell cycle and enter meiosis under nutritionally deficient conditions in Schizosaccharomyces pombe. It was shown that overexpression of Stm1 led growth inhibition and uncontrolled mitotic haploidization presumably by the premature initiation of mitosis. Sty1 and Gpa2 seem to play important roles for Stm1 to deliver starvation signal to induce downstream function. Based on the observation that conversion of diploid to haploid by overexpression of Stm1 can be easily detected as pink or red colonies in the media containing low adenine, HTS drug screening system to identify modulators of GPCR was established and tested using 413 compounds. Four very potent modulators of GPCR including Biochanin A, which possess strong inhibitory activity against uncontrolled cell division, were identified in this screening. This study provides the yeast-based platform that allows robust cellular assays to identify novel modulators of G-protein signaling and MAP kinase pathway.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/j.jbiotec.2007.01.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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