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Title 

Regulatory role of PopN and its interacting partners in type III secretion of Pseudomonas aeruginosa

Authors 

H YangZ ShanJae Wha KimW WuW LianL ZengL XingS Jin

Publisher 

American Society for Microbiology

Issue Date 

2007

Citation 

Journal of Bacteriology, vol. 189, no. 7, pp. 2599-2609

Keywords 

protein popnphenotypepopn genetype III secretion system

Abstract 

The type III secretion system (T3SS) of Pseudomonas aeruginosa plays a significant role in pathogenesis. We have previously identified type III secretion factor (TSF), which is required for effective secretion of the type III effector molecules, in addition to the low calcium signal. TSF includes many low-affinity high-capacity calcium binding proteins, such as serum albumin and casein. A search for the TSF binding targets on the bacterial outer membrane resulted in identification of PopN, a component of the T3SS that is readily detectable on the bacterial cell surface. PopN specifically interacts with Pcr1, and both popN and pcr1 mutants have a constitutive type III secretion phenotype, suggesting that the two proteins form a complex that functions as a T3SS repressor. Further analysis of the popN operon genes resulted in identification of protein-protein interactions between Pcr1 and Pcr4 and between Pcr4 and Pcr3, as well as between PopN and Pcr2 in the presence of PscB. Unlike popN and pcr1 mutants, pcr3 and pcr4 mutants are totally defective in type III secretion, while a pcr2 mutant exhibits reduced type III secretion. Interestingly, PopN, Pcr1, Pcr2, and Pcr4 are all secreted in a type III secretion machinery-dependent manner, while Pcr3 is not. These findings imply that these components have important regulatory roles in controlling type III secretion.

ISSN 

0021-9193

Link 

http://dx.doi.org/10.1128/JB.01680-06

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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