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Title 

Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants

Authors 

Kyoung Sil YangH S KimU H JinS S LeeJ A ParkY P LimH S Pai

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Planta, vol. 225, no. 6, pp. 1459-1469

Keywords 

leaf yellowingnuclear localizationorganellar RNA polymerasesreactive oxygen speciesvirus-induced gene silencingchloroplastgene expression regulationgene silencinggeneticsmitochondrion

Abstract 

BTF3 (βNAC) was originally isolated as a general transcription factor required for RNA polymerase II-dependent transcription, and later found to be a β-subunit of nascent-polypeptide-associated complex that has been implicated in regulating protein localization during translation. In this study, virus-induced gene silencing of NbBTF3 encoding a Nicotiana benthamiana homolog of human BTF3 caused leaf yellowing and abnormal leaf morphology without altering the overall growth of the plant. The NbBTF3 gene is constitutively expressed and the NbBTF3-GFP fusion protein is primarily targeted to the nucleus. At the cellular level, downregulation of NbBTF3 expression reduced the chloroplast sizes and chlorophyll contents. The affected cells produced excessive amounts of reactive oxygen species. Furthermore, the transcript level of various plastid- and mitochondria-encoded genes was severely reduced in the NbBTF3-depleted leaf cells. These findings indicate that depletion of NbBTF3 activity preferentially affected development and/or physiology of chloroplasts and mitochondria in plants, possibly by hampering efficient translocation of the nascent organellar proteins into the organelles.

ISSN 

0032-0935

Link 

http://dx.doi.org/10.1007/s00425-006-0453-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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