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Title 

Identification of potential biomarkers for diazinon exposure to Japanese Medaka (Oryzias latipes) using annealing control primers

 

ACP 기법을 이용한 diazinon에 노출된 송사리의 위해성 바이오마커 발굴

Authors 

J H YooS W ShinJ S KimChang-Bae KimS C Koh

Publisher 

Taylor & Francis

Issue Date 

2007

Citation 

Journal of Environmental Science and Health (Part B), vol. 42, no. 4, pp. 373-379

Keywords 

annealing control primersbiomarkerdiazinondifferentially expressed genesgene ontologymedakareal time PCRbiomarkersidentification methodpollution exposure

Abstract 

A new differential display-polymerase chain reaction (PCR) method based on annealing control primers was used to screen and identify potential biomarkers from differentially expressed genes (DEGs) in medaka exposed to sub-lethal concentration of diazinon (100 ppb). Among the differentially expressed genes identified, the majority were in functional categories of protein biosynthesis, transport and metabolism according to the gene ontology classification. The differential expression of ribosomal protein genes was quantified by real time PCR. The genes encoding ribosomal proteins including L3 and S17 were selected as potential biomarkers for diazinon exposure in medaka fish.

ISSN 

1093-4529

Link 

http://dx.doi.org/10.1080/03601230701310526

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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