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Title 

Protein micropatterning on bifunctional organic-inorganic sol-gel hybrid materials

Authors 

W S KimMin-Gon KimJun Hyoung AhnB S BaeC B Park

Publisher 

American Chemical Society

Issue Date 

2007

Citation 

Langmuir, vol. 23, no. 9, pp. 4732-4736

Keywords 

sol-gelsprotein micropatterningproteinsproteingelprotein microarraygelsprotein array analysis

Abstract 

Active protein micropatterns and microarrays made by selective localization are popular candidates for medical diagnostics, such as biosensors, bioMEMS, and basic protein studies. In this paper, we present a simple fabrication process of thick (∼20 μm) protein micropatterning using capillary force lithography with bifunctional sol-gel hybrid materials. Because bifunctional sol-gel hybrid material can have both an amine function for linking with protein and a methacryl function for photocuring, proteins such as streptavidin can be immobilized directly on thick bifunctional sol-gel hybrid micropatterns. Another advantage of the bifunctional sol-gel hybrid materials is the high selective stability of the amine group on bifunctional sol-gel hybrid patterns. Because amine function is regularly contained in each siloxane oligomers, immobilizing sites for streptavidin are widely distributed on the surface of thick hybrid micropatterns. The micropatterning processes of active proteins using efficient bifunctional sol-gel hybrid materials will be useful for the development of future bioengineered systems because they can save several processing steps and reduce costs.

ISSN 

0743-7463

Link 

http://dx.doi.org/10.1021/la070074p

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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