상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Rapid screen of human genes for relevance to cancer using fission yeast

Authors 

Kyung Sook ChungY J JangNam-Soon KimS Y ParkShin Jung ChoiJ Y KimJ H AhnH J LeeJ H LimS H SongJ H JiJ H OhK B SongHyang Sook YooMi Sun Won

Publisher 

SAGE Publications (UK and US)

Issue Date 

2007

Citation 

Journal of Biomolecular Screening, vol. 12, no. 4, pp. 568-577

Keywords 

cell-based assayfission yeastoncogenesisoverexpressionphenotypecancer cell culturecancer geneticscarcinogenesisgene activitygene expression

Abstract 

A total of 437 human full-length cDNAs isolated by microarray analysis of liver and/or gastric cancer tissues were evaluated for their relevance to cancer using the fission yeast Schizosaccharomyces pombe. Overexpression of 161 human cDNAs in S. pombe caused growth inhibition and/or morphological changes, which can be considered as cancer-related phenotypes of S. pombe. Sixteen genes causing growth defects and morphological changes at the same time were chosen to validate their ostensible oncogenic properties. They were highly expressed in liver and/or gastric cancer cell lines. Also, when the mouse embryonic fibroblast cell type NIH3T3 was transfected with these genes, the proliferation rates of cells were increased by 32% to 120%. This study demonstrates that fission yeast can be used as an advantageous and powerful tool for the rapid screening of human genes relevant to cancer. Furthermore, the human genes screened can be tested further as diagnostic markers and potential therapeutic targets for liver and stomach cancers. They also can be studied further for the elucidation of mechanisms involved in carcinogenesis.

ISSN 

1087-0571

Link 

http://dx.doi.org/10.1177/1087057107301007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)