상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Horseradish peroxidase (HRP) immobilized poly(aniline-co-m-aminophenol) film electrodes-fabrication and evaluation as hydrogen peroxide sensor

Authors 

K D SeoK P LeeA I GopalanSang Jeon ChungYong Taik LimS H Choi

Publisher 

MDPI AG

Issue Date 

2007

Citation 

Sensors, vol. 7, no. 5, pp. 719-729

Keywords 

au nanoparticlescovalent bondinghorseradish peroxidasephysical adsorptionpoly(aniline-co-m-aminophenol)

Abstract 

Enzyme modified electrodes were fabricated with poly(aniline-co-m- aminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymer film through physical entrapment. In the other method, the amino and -OH groups in the copolymer are utilized to form covalent functionalization with HRP via glutaric dialdehyde as cross-linker/mediator. The conducting copolymer/enzyme modified electrodes prepared by physical entrapment/covalent functionalization of enzyme were tested for electrocatalytic activities towards sensing of H2O2. Amperometric results indicate that enzyme modified electrode via physical entrapment possesses better electrocatalytic performance over covalent functionalized enzyme electrode.

ISSN 

1424-8220

Link 

http://dx.doi.org/10.3390/s7050719

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


Files in This Item: SizeFormat
7080.pdf3473KbAdobe PDF
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)