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Title 

Characterization of DNA methylation change in stem cell marker genes during differentiation of human embryonic stem cells

Authors 

Seungeun YeoSangkyun JeongJanghwan KimJ S HanY M HanYong-Kook Kang

Publisher 

Elsevier

Issue Date 

2007

Citation 

Biochemical and Biophysical Research Communications, vol. 359, no. 3, pp. 536-542

Keywords 

DNA methylationhuman embryonic stem cellnanogOct4cell markercell differentiationembryonic stem cellgene locushuman

Abstract 

Pluripotent human embryonic stem cells (hESCs) have the distinguishing feature of innate capacity to allow indefinite self-renewal. This attribute continues until specific constraints or restrictions, such as DNA methylation, are imposed on the genome, usually accompanied by differentiation. With the aim of utilizing DNA methylation as a sign of early differentiation, we probed the genomic regions of hESCs, particularly focusing on stem cell marker (SCM) genes to identify regulatory sequences that display differentiation-sensitive alterations in DNA methylation. We show that the promoter regions of OCT4 and NANOG, but not SOX2, REX1 and FOXD3, undergo significant methylation during hESCs differentiation in which SCM genes are substantially repressed. Thus, following exposure to differentiation stimuli, OCT4 and NANOG gene loci are modified relatively rapidly by DNA methylation. Accordingly, we propose that the DNA methylation states of OCT4 and NANOG sequences may be utilized as barometers to determine the extent of hESC differentiation.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2007.05.120

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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