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Title 

Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip

Authors 

K H LeeHyou-Arm JoungJ H AhnK O KimI S OhYong Beom ShinMin-Gon KimD M Kim

Publisher 

Elsevier

Issue Date 

2007

Citation 

Analytical Biochemistry, vol. 366, no. 2, pp. 170-174

Keywords 

cell-free protein synthesisreal-time monitoringSPRcell free systemchemical reactionincubation timemonitoringpriority journalprotein aggregationprotein analysis

Abstract 

Taking advantage of the "open" nature of cell-free protein synthesis, this study investigated the direct analysis of protein expression using a surface plasmon resonance sensor. During the on-chip incubation of the reaction mixture for cell-free protein synthesis, the expressed protein molecules were immobilized onto the surface of the chip, giving rise to a sensorgram signal, which enabled on-line monitoring of protein expression. In addition, we found that the expression of the aggregation-prone proteins could be effectively monitored. The ability to monitor these proteins was most likely through the instant isolation of the expressed protein molecules onto the solid surface of the chip.

ISSN 

0003-2697

Link 

http://dx.doi.org/10.1016/j.ab.2007.04.044

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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