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Title 

Characterization of levan hydrolysis activity of levansucrase from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071

Authors 

K H JangS A KangChul Ho KimJ C LeeM H KimE SonSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Food Science and Biotechnology, vol. 16, no. 3, pp. 482-484

Keywords 

hydrolysis of levanlevanlevansucrasetransfructosylationrahnella aquatiliszymomonas mobilis

Abstract 

To investigate production and hydrolysis of levan, the levansucrase enzymes from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071 were used. The optimum temperature of R. aquatilis levansucrase for levan formation was 37°C, whereas that of Z. mobilis was 4°C, under the experimental conditions. Both levansucrases also catalyzed the reverse levan hydrolysis reaction. Levan hydrolysis reactions from both levansucrases were temperature dependent; high temperature (20°C) was more favorable than low temperature (4°C) by 4 times. Fructose was the only product from hydrolysis reaction by both levansucrases, showing that both levansucrases mediated the hydrolysis reaction of exo-enzyme acting. In both enzymes, initial levan hydrolysis activity was almost accounted to 1% of initial levan formation activity. The results allow the estimation of the fructose release rate in enzyme processing conditions.

ISSN 

1226-7708

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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