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Title 

Phosphoproteomic analysis of neuronal cell death by glutamate-induced oxidative stress

 

글루타메이트유도 산화적 스트레스에 의한 신경세포의 인산화 단백질체 연구

Authors 

T H KangKwang-Hee BaeM J YooWon Kon KimH R HwangHye-Yun JungPhil Young LeeSunghyun KangTae-Sung YoonSung Goo ParkSeong Eon RyuSang Chul Lee

Publisher 

Wiley-Blackwell

Issue Date 

2007

Citation 

Proteomics, vol. 7, no. 15, pp. 2624-2635

Keywords 

apoptosisHT22oxidative stressphosphoproteomeROSanimal cellcell culturecell deathcell linenerve cell necrosis

Abstract 

Oxidative stress is one of the major causes of neuronal cell death in disorders such as perinatal hypoxia and ischemia. Protein phosphorylation is the most significant PTM of proteins and plays an important role in stress-induced signal transduction. Thus, the analysis of alternative protein phosphorylation states which occur during oxidative stress-induced cell death could provide valuable information regarding cell death. In this study, a reference phosphoproteome map of the mouse hippocampal cell line HT22 was constructed based on 125 spots that were identified by MALDI-TOF or LC-ESI-Q-TOF-MS analysis. In addition, proteins of HT22 cells at various stages of oxidative stress-induced cell death were separated by 2-DE and alterations in phosphoproteins were detected by Pro-Q Diamond staining. A total of 17 spots showing significant quantitative changes and seven newly appearing spots were identified after glutamate treatment. Splicing factor 2, peroxiredoxin 2, S100 calcium binding protein A11, and purine nucleoside phosphorylase were identified as up- or down-regulated proteins. CDC25A, caspase-8, and cyp51 protein appeared during oxidative stress-induced cell death. The data in this study from phosphoproteomic analysis provide a valuable resource for the understanding of HT22 cell death mechanisms mediated by oxidative stress.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200601028

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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