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Title 

Oral administration of high molecular mass poly-γ-glutamate induces NK cell-mediated antitumor immunity

Authors 

T W KimTae Young LeeH C BaeJ H HahmYang Hyun KimC ParkT H KangC J KimM H SungHaryoung Poo

Publisher 

American Association of Immunologists

Issue Date 

2007

Citation 

Journal of Immunology, vol. 179, no. 2, pp. 775-780

Keywords 

oral drug administrationadministration, oral

Abstract 

We analyzed the in vivo tumor regression activity of high molecular mass poly-gamma-glutamate (gamma-PGA) from Bacillus subtilis sups. chungkookjang. C57BL/6 mice were orally administered 10-, 100-, or 2000-kDa gamma-PGA or beta-glucan (positive control), and antitumor immunity was examined. Our results revealed higher levels of NK cell-mediated cytotoxicity and IFN-gamma secretion in mice treated with higher molecular mass gamma-PGA (2000 kDa) vs those treated with lower molecular mass gamma-PGA (10 or 100 kDa) or beta-glucan. We then examined the effect of oral administration of 10- or 2000-kDa gamma-PGA on protection against B16 tumor challenge in C57BL/6 mice. Mice receiving high molecular mass gamma-PGA (2000 kDa) showed significantly smaller tumor sizes following challenge with the MHC class I-down-regulated tumor cell lines, B16 and TC-1 P3 (A15), but not with TC-1 cells, which have normal MHC class I expression. Lastly, we found that gamma-PGA-induced antitumor effect was decreased by in vivo depletion of NK cells using mAb PK136 or anti-asialo GM1 Ab, and that was completely blocked in NK cell-deficient B6 beige mice or IFN-gamma knockout mice. Taken together, we demonstrated that oral administration of high molecular mass gamma-PGA (2000 kDa) generated significant NK cell-mediated antitumor activity in mice bearing MHC class I-deficient tumors.

URI 

https://doi.org/10.4049/jimmunol.179..775

ISSN 

0022-1767

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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