상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor

Authors 

H A JoungW B ShimD H ChungJun Hyoung AhnBong Hyun ChungH S ChoiS D HaK S KimK H LeeC H KimK Y KimMin-Gon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Biotechnology and Bioprocess Engineering, vol. 12, no. 2, pp. 80-85

Keywords 

antibodylisteria monocytogenesprotein Lsurface plasmon resonance (SPR)aminebacterium antibodycarboxymethyldextrandihydrolipoamide dehydrogenasemonoclonal antibodyantigen binding

Abstract 

In this study, a specific monoclonal antibody against Listeria monocytogenes was screened using an SPR biosensor. Monoclonal antibodies were bound to protein L, after which the L. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an arnine coupling method, and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection of L. monocytogenes. Under optimized conditions, 103 cells/mL or 50 cells were detected by the SPR biosensor.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/BF03028630

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)