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Title 

In vitro biosynthesis of strictosidine using Lonicera japonica leaf extracts and recombinant yeast

Authors 

K H NamH J ChungE J JeonM K ParkY H YimJang Ryol LiuJ H Park

Publisher 

The Botanical Society of Korea

Issue Date 

2007

Citation 

Journal of Plant Biology, vol. 50, no. 3, pp. 315-320

Keywords 

catharanthus roseushoneysuckle lonicera japonicasecologaninstrictosidinestrictosidine synthaseloniceralonicera japonica

Abstract 

Strictosidine is a key intermediate in the biosynthesis of the terpenoid indole alkaloid (TIA) pathway. It results from a condensation reaction, catalyzed by strictosidine synthase (STR), between tryptamine and secologanin. We have now developed a useful method, based on enzyme-assisted synthesis, to produce strictosidine. Our procedure utilizes leaf extracts from Japanese honeysuckle Lonicera japonica Thunb. as a secologanin source. In these experiments, an enzyme extract was prepared from transgenic yeast Saccharomyces cerevisiae that expresses the Catharanthus roseus STR (CrSTR) coding region. Strictosidine was then isolated with a 38% yield based on the initial amount of tryptamine in the enzymatic reaction.

ISSN 

1226-9239

Link 

http://dx.doi.org/10.1007/BF03030660

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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