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Title | Plant regeneration from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis |
Authors | Sung Ran Min; Jang Ryol Liu; Suk Weon Kim |
Publisher | Springer Verlag (Germany) |
Issue Date | 2007 |
Citation | Plant Biotechnology Reports, vol. 1, no. 1, pp. 57-60 |
Keywords | ranunculus kazusensis; plant regeneration; somatic embryogenesis; suspension culture |
Abstract | Culture conditions for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Ranunculus kazusensis are described. Zygotic embryos formed white nodular structures and pale-yellow calluses at a frequency of 84.9% when cultured on half-strength Schenk and Hildebrandt (SH) medium supplemented with 0.1 mg l.1 2,4-dichlorophenoxyacetic acid (2,4-D). However, the frequency of white nodular structure and off-white callus formation decreased with an increasing concentration of 2,4-D up to 10 mg l.1, when the frequency reached 25%. Cell suspension cultures were established from zygotic embryo-derived pale-yellow calluses using half-strength SH medium supplemented with 0.1 mg l.1 of 2,4-D. Upon plating onto halfstrength SH basal medium, over 90% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted to potting soil and grown to maturity at a survival rate of over 90% in a growth chamber. The plant regeneration system established in this study can be applied to mass propagation and conservation of this species. |
ISSN | 1863-5466 |
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Registered Date |
2017-04-19 |
