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Title 

Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing

Authors 

K J KimJ H LimSanghyeob LeeY J KimS B ChoiM K LeeD ChoiK H Paek

Publisher 

Elsevier

Issue Date 

2007

Citation 

Biochemical and Biophysical Research Communications, vol. 362, no. 3, pp. 554-561

Keywords 

capsicum annuumfatty acid desaturaseTMVvirus-induced gene silencingfatty acid desaturase 1DNA microarraygene silencingmicroarray analysistobacco mosaic virusvirus induction

Abstract 

A series of microarray analyses employing the expressed sequence tags (ESTs) of hot pepper was conducted in an effort to elucidate the molecular mechanisms inherent to hypersensitive response (HR) by viral or bacterial pathogens. There were 2535 ESTs exhibiting differential expression (over 2-fold changes) among about 5000 ESTs during viral or bacterial response. Further, via virus-induced gene silencing (VIGS) and TMV-infection studies, we were able to isolate several ESTs, which may be relevant to defense response against TMV. of these ESTs, Capsicum annuum fatty acid desaturase 1 (CaFAD1) showed the distinct phenotype against TMV infection and thus was subjected to further study. CaFAD1-silenced plants showed weaker resistance against TMV-P0 infection compared to TRV2 control plants. Also the suppression of FAD1 expression caused blocking of cell death induced by Bcl2-associated X (Bax) protein in tobacco plants. Therefore, this report presents that both microarray and VIGS approaches are feasible in hot pepper plants and the TMV-induced CaFAD1 plays a role in HR response.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2007.07.095

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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