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Title 

Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems

Authors 

Jeong Nam ParkMin Jeong SohnDoo-Byoung OhOh Suk KwonSang Ki RheeCheol-Goo HurS Y LeeG GellissenHyun Ah Kang

Publisher 

American Society for Microbiology

Issue Date 

2007

Citation 

Applied and Environmental Microbiology, vol. 73, no. 19, pp. 5990-6000

Keywords 

cadmiumgene expressionmercury (metal)heavy metal contaminationmethylotrophic yeast Hansenula polymorphawhole-cell biosensorsheavy metaltranscriptomegene deletiongene expression profiling

Abstract 

The genomewide gene expression profiling of the methylotrophic yeast Hansenula polymorpha exposed to cadmium (Cd) allowed us to identify novel genes responsive to Cd treatment. To select genes whose promoters can be useful for construction of a cellular Cd biosensor, we further analyzed a set of H. polymorpha genes that exhibited >6-fold induction upon treatment with 300 μM Cd for 2 h. The putative promoters, about 1,000-bp upstream fragments, of these genes were fused with the yeast-enhanced green fluorescence protein (GFP) gene. The resultant reporter cassettes were introduced into H. polymorpha to evaluate promoter strength and specificity. The promoter derived from the H. polymorpha SEO1 gene (HpSEO1) was shown to drive most strongly the expression of GFP upon Cd treatment among the tested promoters. The Cd-inducible activity was retained in the 500-bp deletion fragment of the HpSCO1 promoter but was abolished in the further truncated 250-bp fragment. The 500-bp HpSEO1 promoter directed specific expression of GFP upon exposure to Cd in a dose-dependent manner, with Cd detection ranging from 1 to 900 μM. Comparative analysis of the Saccharomyces cerevisiae SEO1 (ScSEO1) promoter revealed that the ScSEO1 promoter has a broader specificity for heavy metals and is responsive to arsenic and mercury in addition to Cd. Our data demonstrate the potential use of the HpSEO1 promoter as a bioelement in whole-cell biosensors to monitor heavy metal contamination, particularly Cd.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.00863-07

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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