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Title 

Relation of poly(ADP-ribose) polymerase cleavage and apoptosis induced by paclitaxel in HeLa S3 uterine cancer cells

 

HeLa S3 자궁암 세포에서 paclitaxel에 의해 유도된 poly(ADP-ribose) polymerase 분철

Authors 

J H ChangK Y KimMoon Soo RheeS C AhnH Y Kwon

Publisher 

Korean Society of Life Science

Issue Date 

2007

Citation 

Korean Journal of Life Sciences, vol. 17, no. 8, pp. 1027-1033

Keywords 

paclitaxelPARPBcl-2

Abstract 

Although paclitaxel induces apoptosis of cancer cells, its exact mechanism of action is not yet known. The present study has been performed to determine whether influence of paclitaxel in HeLa S3 uterine cancer cells. Three assays were employed in this study: cell cytotoxicity, morphological assessments of apoptotic cells (DAPI staining assay), and western blot analysis. The results indicated that paclitaxel has cytotoxic effects in HeLa S3 cells. Especially, the IC50 value of paclitaxel was about 1 μM. And morphological changes (fragmentation) of cells were observed by paclitaxel in HeLa S3 cells. The flow cytometric analysis of paclitaxel-treated cells indicated a block of G2/M phase. The results that paclitaxel regulates the cell cycle, especially Sub-G1 phase. Paclitaxel induces apoptosis of HeLa S3 cells via PARP-dependent fashion, and this apoptosis is related to disappearance of Bcl-2 proteins.

ISSN 

1225-9918

Link 

http://dx.doi.org/10.5352/JLS.2007.17.8.1027

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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