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Title 

Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia

 

Eosinophilia 모델로서의 AML14.3D10 세포주의 인산화단백질체 연구

Authors 

Soo In RyuWon Kon KimH J ChoPhil Young LeeH JungTae-Sung YoonJeong Hee MoonSunghyun KangHaryoung PooKwang-Hee BaeSang Chul Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Journal of Biochemistry and Molecular Biology, vol. 40, no. 5, pp. 765-772

Keywords 

AML14.3D10atopic dermatitiseosinophiliaphosphoproteomedexamethasonecell linefactual databasehumanmetabolism

Abstract 

Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.

ISSN 

1225-8687

Link 

http://dx.doi.org/10.5483/BMBRep.2007.40.5.765

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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