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Title 

Expression and promoter analyses of pepper CaCDPK4 (Capsicum annuum calcium dependent protein kinase 4) during plant defense response to incompatible pathogen

 

고추 CDPK4 유전자의 발현 및 프로모터

Authors 

E ChungS K OhJeong Mee ParkD Choi

Publisher 

Han Rim Won Publishing Company

Issue Date 

2007

Citation 

Plant Pathology Journal, vol. 23, no. 2, pp. 76-89

Keywords 

CDPKchili peppernon-host resistancepromoter

Abstract 

CaCDPK4, a full-length cDNA clone encoding Capsicum annuum calcium-dependent protein kinase 4, was isolated from chili pepper (Capsicum annuum L.). Deduced amino acid sequence of CaCDPK4 shares the highest homology with tobacco NpCDPK8 and chickpea CaCDPK2 with 79% identity. Genomic blot analyses revealed that CaCDPK4 is present as a single copy in pepper genome, but it belongs to a multigene family. CaCDPK4 was highly induced when pepper plants were inoculated with an incompatible bacterial pathogen. Induced levels of CaCDPK4 transcripts were also detected in pepper leaves by the treatment of ethephon, an ethylene-inducing agent, and high-salt stress condition. The bacterial-expressed GST-CaCDPK4 protein showed to retain the autophosphorylation activity in vitro. GUS expression driven by CaCDPK4 promoter was examined in transgenic Arabidopsis containing transcriptional fusion of CaCDPK4 promoter. GUS expression under CaCDPK4 promoter was strong in the root and veins of the seedlings. GW (-1965) and D3 (-1377) promoters conferred on GUS expression in response to inoculation of an incompatible bacterial pathogen, but D4-GUS (-913) and D5-GUS (-833) did not. Taken together, our results suggest that CaCDPK4 can be implicated on signal transduction pathway of defense response against an incompatible bacterial pathogen in pepper.

ISSN 

1598-2254

Link 

http://dx.doi.org/10.5423/PPJ.2007.23.2.076

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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