상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Identification and molecular modeling of a family 5 endocelluase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus

Authors 

Dooil KimB H ParkB W JungM K KimS I HongDae Sil Lee

Publisher 

MDPI AG

Issue Date 

2007

Citation 

International Journal of Molecular Sciences, vol. 7, no. 12, pp. 571-589

Keywords 

endocellulasethermus caldophilus GK24glycosyl hydrolase family 5homology modelingmolecular dynamicscongo-red staining

Abstract 

The genome of T. caldophilus GK24 was recently sequenced and annotated as 14 contigs, equivalent to 2.3 mega basepairs (Mbp) of DNA. In the current study, we identified a unique 13.7 kbp DNA sequence, which included the endocellulase gene of T. caldophilus GK24, which did not appear to be present in the complete genomic sequence of the closely related species T. thermophilus HB27 and HB8. Congo-red staining revealed a unique phenotype of cellulose degradation by strain GK24 that was distinct from other closely related Thermus strains. The results showed that strain GK24 is an aerobic, thermophilic, cellulolytic eubacterium which belongs to the group T. thermophilus. In order to understand the mechanism of production of cellobiose in T. caldophilus GK24, a three-dimensional model of the endocellulase, TcCel5A, was generated based on known crystal structures. Using this model, we carried out a flexible cellotetraose docking study.

ISSN 

1422-0067

Link 

http://dx.doi.org/10.3390/i7120571

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


Files in This Item: SizeFormat
7214.pdf1421KbAdobe PDF
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)