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Title 

Caspase-7 mediated cleavage of proteasome subunits during apoptosis

 

세포사멸과정 동안의 caspase-7에 의한 프로테아좀 구성체들의 cleavage

Authors 

Mi JangByoung Chul ParkA Y LeeK S NaSunghyun KangKwang-Hee BaeP K MyungB C ChungS ChoDo Hee LeeSung Goo Park

Publisher 

Elsevier

Issue Date 

2007

Citation 

Biochemical and Biophysical Research Communications, vol. 363, no. 2, pp. 388-394

Keywords 

2-DEapoptosiscaspase-7proteasomeubiquitination26S proteasomeproteasome alpha2proteasome alpha6cell strain MCF 7enzyme subunit

Abstract 

Caspase-3 and caspase-7 are structurally closely related and demonstrate overlapping substrate specificity. However, during apoptosis, they are differentially regulated and show distinct subcellular localizations, implying the presence of specific substrates. In this study, to identify caspase-7 substrates, we treated the lysates derived from caspase-3-deficient MCF-7 cells with purified caspase-7 and analyzed decreased proteins by 2-DE. Intriguingly, several proteasome subunits such as α2, α6, and Rpt1 are degraded by caspase-7 during apoptosis in vitro and in vivo. Caspase-7 mediated cleavage of proteasome subunits results in the reduction of proteasome activity and thereby increases the accumulation of ubiquitinated proteins in cells. These findings suggest that caspase-7 facilitates the execution of apoptosis through down-regulation of the 26S proteasome, which regulates the turnover of proteins involved in the apoptotic process.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2007.08.183

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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