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Title 

Dynamic DNA methylation reprogramming: Active demethylation and immediate remethylation in the male pronucleus of bovine zygotes

Authors 

Jung Sun ParkYoung Sun JeongS T ShinKyung Kwang LeeYong-Kook Kang

Publisher 

Wiley-Blackwell

Issue Date 

2007

Citation 

Developmental Dynamics, vol. 236, no. 9, pp. 2523-2533

Keywords 

active demethylationbovine embryode novo methylationDNA methylationepigenetichistone methylationmale pronucleusreprogrammingdemethylationdna demethylation

Abstract 

DNA methylation reprogramming (DMR) is believed to be a key process by which mammalian zygotes gain nuclear totipotency through erasing epigenetic modifications acquired during gametogenesis. Nonetheless, DMR patterns do not seem to be conserved among mammals. To identify uniform rules underlying mammalian DMRs, we explored DMRs of diverse mammalian zygotes. of the zygotes studied, of particular interest was the bovine zygote; the paternal DNA methylation first decreased and was then rapidly restored almost to the maternal methylation level even before the two-cell stage. The 5-azadeoxycytidine treatment led to complete demethylation of the male pronucleus. The unusually dramatic changes in DNA methylation levels indicate that the bovine male pronucleus undergoes active demethylation, which is followed by de novo methylation. Our results show that, in bovine, the compound processes of active DNA demethylation and de novo DNA methylation, along with de novo H3-K9 trimethylation also, take place altogether within this very narrow window of pronucleus development.

ISSN 

1058-8388

Link 

http://dx.doi.org/10.1002/dvdy.21278

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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