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Title 

Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media

Authors 

T L M StamfordT C M StamfordN P StamfordC E R S SantosM C C P LyraY H ParkJin-Woo BaeJ M Araujo

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

World Journal of Microbiology & Biotechnology, vol. 23, no. 12, pp. 1719-1724

Keywords 

α-Amylase16S rDNAamyloglucosidaseamylolitic enzymesgene sequencepachyrhizus erosuskitasatospora recifensis

Abstract 

An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher α-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817 T = KCTC 9972T= DSM 44943T).

ISSN 

0959-3993

Link 

http://dx.doi.org/10.1007/s11274-007-9420-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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