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Title 

Over-expression of c-raf-1 proto-oncogene in liver cirrhosis and hepatocellular carcinoma

Authors 

Y H HwangJ Y ChoiS KimE S ChungT KimSang Seok KohB LeeS H BaeJ KimY M Park

Publisher 

Wiley-Blackwell

Issue Date 

2004

Citation 

Hepatology Research, vol. 29, no. 2, pp. 113-121

Keywords 

hepatocarcinogenesishepatocellular carcinomaliver cirrhosisMAPK pathwayRaf-1ribosomal protein S6complementary DNAmitogen activated protein kinaseRaf proteincontrolled study

Abstract 

Liver cirrhosis accompanies at least 70% of hepatocellular carcinomas world-wide. To evaluate the dysregulation of apoptosis and the MAPK pathway in hepatocarcinogenesis, we investigated the expression profiles of the genes involved in apoptosis and MAPK pathway in cirrhosis and hepatocellular carcinoma. A total of 94 tissue specimens (61 cirrhosis and 33 hepatocellular carcinoma) obtained from 67 patients were analyzed by microarray, quantitative PCR and Western blot experiments.Of 71 apoptosis-associated genes, c-raf -1 and S6 were up-regulated in 42.9% and 32.1% of 28 cirrhosis tissues, respectively, and both genes were well correlated in a five-cluster K -means analysis. For c-raf -1 and down stream genes in the MAPK pathway, c-raf -1, MEK, and MAPK were up-regulated in 40%, 80%, and 86.7% of 45 cirrhosis specimens, respectively, and in 50%, 63.6%, and 59.1% of 22 hepatocellular carcinoma specimens, respectively. Western blot analysis showed that activated Raf-1 was over-expressed in 91.2% (52/57) of cirrhosis and in 100% (30/30) of hepatocellular carcinoma. The expression level of Raf-1 in 14 of 26 paired samples (53.8%) was significantly higher in hepatocellular carcinoma than in cirrhosis (16.2±20.3-fold, P<0.05). These results suggest that the activation of Raf-1 plays an important role in the development of hepatocellular carcinoma.

ISSN 

1386-6346

Link 

http://dx.doi.org/10.1016/j.hepres.2004.02.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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