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Title 

Two step carcinogen screening model using neonatal FVB/NJ mouse

Authors 

Young Suk WonHyo Jung KwonY K ChoiH J ParkE S JeongBon Chul KooOg Sung MoonKi Hoan NamHyoung-Chin Kim

Publisher 

Korean Association for Laboratory Animal Science

Issue Date 

2007

Citation 

Labolatory Animal Research, vol. 23, no. 3, pp. 239-243

Keywords 

FVB/NJcarcinogenesishepatocellular adenoma2-acetlyaminofluorene (2-AAF)

Abstract 

A long-term carcinogenicity study is required to assess the safety of new drug candidates. This classical toxicological method has been using rats and mice as animal systems. The International Conference on Harmonization (ICH) of technical requirements of pharmaceuticals for human use have recommended one long-term rodent carcinogenicity test using rat and one other supplementary mouse study such as an initiation-promotion model, a transgenic model or a neonatal model. This study was carried out to develop an initiation-promotion liver carcinogenicity model using a mouse. Twelve-day old FVB/NJ mice were intraperitoneally injected with diethyl nitrosamine (DEN) as an initiator and orally administered with 2-acetlyaminofluorene (2-AAF) as a promoter. And D-galactosamine (DGA) was injected as a stimulator for tumorigenic progress. Basophilic foci in the liver were observed 16 weeks after initiation. The incidence of hepatocellular adenoma was increased in animals 2-AAF-treated at 20 and 26 weeks after initiation. The tumor incidence in 2-AAF-treated animals were significantly higher than with the untreatment and initiator treatment control groups. Hepatocellular carcinoma (HCC) was found in some cases. These results suggest that two-step neonatal FVB/NJ mouse model could be useful for carcinogenic assay of new drug candidates.

ISSN 

1738-6055

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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