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Title 

Genetic transformation of Chlamydomonas reinhardtii with the RNAi suppressor p19 gene of tombus virus

 

Tombus 바이러스의 RNAi suppressor p19 유전자에 의한 Chlamydomonas reinhardtii의 형질전환

Authors 

Won Joong ChungJang Ryol LiuH Cerutti

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2007

Citation 

Journal of Plant Biotechnology, vol. 34, no. 4, pp. 307-312

Abstract 

Chlamydomonas reinhardtii was transformed with the coding sequence of the Tombus virus gene p19 to determine whether the gene functions as an RNAi suppressor in C. reinhardtii. Transformants were confirmed to have 1 to several copies of p19 gene in their chromosomes. When an RNAi strain of C. reinhardtii generated by transforming the inverted repeat (IR) sequence homologous to the 3’UTR region of the MAA7 gene was retransformed with the gene p19, MAA7 transcript levels of transformants fluctuated and proliferation of transformants on the medium containing 5-FI was suppressed. Overall results suggest that p19-mediated silencing suppression works at a low level in C. reinhardtii because of difference in codon usage resulting in weak P19 expression unless p19-mediated silencing suppression in C. reinhardtii works in a different manner from higher plants.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2007.34.4.307

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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